Dr. Oz hearts GSH

Love me some Dr. Oz.

More support for our friend NAC, precursor to Glutathione (GSH).

CFers are known for lacking dearly in GSH....   The Superhero of Antioxidants, Pt 1.

I would love hear from anyone who has gotten IV GSH. Never heard of it before this segment.

The effect of N-acetylcysteine on chloride efflux from airway epithelial cells

Cell Biol Int. 2009 Oct 20

The effect of N-acetylcysteine on chloride efflux from airway epithelial cells.

Varelogianni G, Oliynyk I, Roomans GM, Johannesson M.

Defective chloride transport in epithelial cells increases mucus viscosity and leads to recurrent infections with high oxidative stress in patients with cystic fibrosis (CF). N-acetylcysteine (NAC) is a well known mucolytic and antioxidant drug, and an indirect precursor of glutathione.

Since S-nitrosoglutathione (GSNO) previously has been shown to be able to promote Cl- efflux from CF airway epithelial cells, it was investigated whether NAC also could stimulate Cl- efflux from CF and non-CF epithelial cells and through which mechanisms.

CF bronchial epithelial cells (CFBE) and normal bronchial epithelial cells (16HBE) were treated with 1mM, 5mM, 10mM or 15mM NAC for 4h at 37 degrees C. The effect of NAC on Cl- transport was measured by Chloride efflux measurements and by X-ray microanalysis. Cl- efflux from CFBE cells was stimulated by NAC in a dose dependent manner, with 10mM NAC causing a significant increase in Cl- efflux with nearly 80% in CFBE cells.

The intracellular Cl- concentration in CFBE cells was significantly decreased up to 60 % after 4h treatment with 10mM NAC. Moreover immunocytochemistry and Western Blot experiments revealed expression of CFTR channel on CFBE cells after treatment with 10mM NAC.

The stimulation of Cl- efflux by NAC in CF airway epithelial cells may improve hydration of the mucus and thereby be beneficial for CF patients.

Whey Protein and GSH

Thanks to @immuneprotect on Twitter for tweeting this article

: J Cyst Fibros. 2003 Dec;2(4):195-8.

Erratum in:

J Cyst Fibros. 2004 Mar;3(1):62.

Improved glutathione status in young adult patients with cystic fibrosis supplemented with whey protein.

Grey V, Mohammed SR, Smountas AA, Bahlool R, Lands LC.

The Department of Pathology and Molecular Medicine, McMaster Division, Hamilton Health Sciences, Hamilton, Ontario, Canada.

BACKGROUND: The lung disease of cystic fibrosis is associated with a chronic inflammatory reaction and an over abundance of oxidants relative to antioxidants. Glutathione functions as a major frontline defense against the build-up of oxidants in the lung. This increased demand for glutathione (GSH) in cystic fibrosis may be limiting if nutritional status is compromised. We sought to increase glutathione levels in stable patients with cystic fibrosis by supplementation with a whey-based protein.

METHODS: Twenty-one patients who were in stable condition were randomly assigned to take a whey protein isolate (Immunocal, 10 g twice a day) or casein placebo for 3 months. Peripheral lymphocyte GSH was used as a marker of lung GSH. Values were compared with nutritional status and lung parameters.

RESULTS: At baseline there were no significant differences in age, height, weight, percent ideal body weight or percent body fat. Lymphocyte GSH was similar in the two groups. After supplementation, we observed a 46.6% increase from baseline (P <>

ONCLUSION: The results show that dietary supplementation with a whey-based product can increase glutathione levels in cystic fibrosis. This nutritional approach may be useful in maintaining optimal levels of GSH and counteract the deleterious effects of oxidative stress in the lung in cystic fibrosis. Copyright 2003 European Cystic Fibrosis Society

PMID: 15463873 [PubMed - indexed for MEDLINE]

Related articles

• Lymphocyte glutathione levels in children with cystic fibrosis.

  Chest. 1999 Jul; 116(1):201-5.

Glutathione exhibits antibacterial activity and increases tetracycline efficacy against Pseudomonas aeruginosa

Thanks so much to dasjsmum for providing us with the link to the full article! :)

http://www.scichina.com:8082/sciCe/EN/article/downloadArticleFile.do?attachType=PDF&id=413350

YaNi Zhang¹ and KangMin Duan¹

(1)	Molecular Microbiology Laboratory, the College of Life Sciences, Northwest University, Xi’an, 710069, China

Received: 5 February 2009 Accepted: 5 March 2009 Published online: 26 June 2009

Abstract Glutathione (GSH) plays important roles in pulmonary diseases, and inhaled GSH therapy has been used to treat cystic fibrosis (CF) patients in clinical trials. The results in this report revealed that GSH altered the sensitivity of Pseudomonas aeruginosa to different antibiotics through pathways unrelated to the oxidative stress as generally perceived. In addition, GSH and its oxidized form inhibited the growth of P. aeruginosa.

Keywords glutathione - antibiotics - Pseudomonas aeruginosa - tetracycline - cystic fibrosis

Supported by the National Natural Science Foundation of China (Grant Nos. 30870097 and 30611120520)

PARTIAL TEXT:
http://www.springerlink.com/content/x2062p64w10683w8/fulltext.pdf?page=1

*I wish I had the full text of this article. Contact your CF Center or Pulmo to see if they subscribe to this journal*